Journal: Cell Death & Disease

Article Title: Direct conversion of human umbilical cord mesenchymal stem cells into retinal pigment epithelial cells for treatment of retinal degeneration

doi: 10.1038/s41419-022-05199-5

Figure Lengend Snippet: Pigment generation, RPE-specific and EMT-associated markers in iPSC-RPE cells, iRPE cells, and ARPE19 cells were detected by A pigment generation and immunostaining, B Western blotting and C quantitative analysis ( n = 3). D The Pearson correlation coefficient matrix of all samples based on RNA-seq datasets. E Electron micrographs of iPSC-RPE cells, iPRE cells, and ARPE19 cells. F Total bound and phagocyted POSs (pointed by arrows) in iPSC-RPE cells, iRPE cells, and ARPE19 cells. G Quantification of phagocytosis, as determined by the number of bound and phagocyted POS per field ( n = 8). H Tight junction were demonstrated by ZO-1 immunostaining. I TER analysis ( n = 4). J HRP permeability assay ( n = 4). K Expression levels of PEDF and VEGF from upper and lower chambers were determined by ELISA ( n = 4). Scale bar = 50 μm. Results are expressed as mean ± SD. P value measured by one-way ANOVA and post hoc Bonferroni’s test.

Article Snippet: PEDF and VEGF were quantified by PEDF ELISA kit (Elabscience, Wuhan, China) and VEGF ELISA kit (Proteintech).

Techniques: Immunostaining, Western Blot, RNA Sequencing Assay, Permeability, Expressing, Enzyme-linked Immunosorbent Assay

Journal: Cell Death & Disease

Article Title: Direct conversion of human umbilical cord mesenchymal stem cells into retinal pigment epithelial cells for treatment of retinal degeneration

doi: 10.1038/s41419-022-05199-5

Figure Lengend Snippet: A Experiment design for iRPE transplantation in SI-induced rat AMD model. B ERG waveforms recorded at different time points (the calibration indicates 200 μV vertically and 25 ms horizontally). C Quantitative analysis of ERG b-wave amplitude ( n = 10, P value measured by one-way ANOVA and post hoc Bonferroni’s test). D Representative micrographs of retinal samples at week 6 post-transplantation. The injection sites were pointed by arrows, and ONL was between yellow dashed lines. E Quantitative analysis of ONL thickness (μm) ( n = 6, P value measured by one-way ANOVA and post hoc Bonferroni’s test). F Representative micrographs of retinal cryosections stained with TUNEL. G Statistical analysis of the percentage of the apoptotic cells in ONL ( n = 6, P value measured by one-way ANOVA and post hoc Bonferroni’s test). H Immunostaining of hUCMSCs and iRPE cells after transplantation in vivo for 4 weeks. I Quantitative analysis of percent of immunostaining+ cells of grafted cells ( n = 7, P value measured by Student’s unpaired t test). J The level of PEDF secreted by hUCMSCs and iRPE cells were determined by ELISA ( n = 4, P value measured by Student’s unpaired t test). Scale bar = 50 μm. Results are expressed as mean ± SD; ** P < 0.01, *** P < 0.001, compared with PBS group; # P < 0.05, ## P < 0.01 compared with hUCMSC group; $ P < 0.05 compared with PBS group.

Article Snippet: PEDF and VEGF were quantified by PEDF ELISA kit (Elabscience, Wuhan, China) and VEGF ELISA kit (Proteintech).

Techniques: Transplantation Assay, Injection, Staining, TUNEL Assay, Immunostaining, In Vivo, Enzyme-linked Immunosorbent Assay

Journal: bioRxiv

Article Title: Effect of flavonoids hydroxygenkwanin on vascular smooth muscle cell proliferation, migration, and neointimal formation

doi: 10.1101/2022.12.20.521220

Figure Lengend Snippet: (A) The number of circulating blood EPCs was determined by flow cytometry 5 days after endothelial denudation (ED). Treatment with HGK (1 mg/kg) increased the number of circulating EPCs after ED. (B) Transwell assays showed that HGK promoted EPC migration. (C) Denuded femoral arteries were stained with Evans blue dye 7 days after ED. Femoral artery staining demonstrated that HGK promoted reendothelialization. (D) Immunohistochemical staining for CD31 was performed. HGK treatment increased CD31 expression (red) in denuded femoral arteries at 28 days after ED. Nuclei were stained with DAPI or hematoxylin. The thickness of neointimal hyperplasia is indicated by the double-headed arrows. The values are presented as the means ± SDs. *p <0.05 vs. the ED/CTRL or CTRL group. N=5-12. CTRL, control group. Statistical comparisons were performed using Student’s t test and one-way ANOVA.

Article Snippet: Human EPCs were obtained from CELLvo™ Human Endothelial Progenitor Cells (StemBioSys, San Antonio, TX) and then cultured in EGM-2 medium.

Techniques: Flow Cytometry, Migration, Staining, Immunohistochemical staining, Expressing